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. 2019 Sep 30;27(4):1398–1414. doi: 10.1038/s41418-019-0426-2

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© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Localization of PCD cells in E12.5 and 13.5 mouse hearts. a, b Sagittal sections of a transgenic heart at E12.5. Magnification of the boxed area in a: bright YFP⁺ signals (white arrows) mark PCD cells or PCD bodies b. c Frontal sections of a transgenic heart at E13.5. Labeled sA5-YFP PCD cells and PCD bodies (white arrows) were found in the trabeculae of both ventricles, the developing mitral valve, and the right atria. d, e Magnifications of boxed areas in a depicting the presence of PCD (sA5-YFP, arrows). V ventricle, RA right atrium, LA left atrium, Tr trabeculae, DTV developing tricuspid valve, and DVM developing mitral valve. Bars are 200 µm (a, c) and 20 µm (b, d, e)