Fig. 5.

DAT regulates cellular iron homeostasis via IRP-IRE signaling. a The structure of IRE-driven fluorescent reporters based on ferritin. b Quantitative evaluation of percentage luciferase/GFP and mCherry/GFP regulated by the combination of DAT and erastin in MEF cells for 6 h. Values are expressed as a percentage change from untreated controls. c Fluorescence microscopy images of mCherry and GFP in MEFs cells expressed with the mCherry/GFP reporter for 6 h and treated with DAT and/or erastin. d Schematic showing the oxidation of arachidonic acid catalyzed by ferrous ion and ascorbate. e DAT does not affect lipid peroxidation in the ferrous-ascorbate catalytic system. Plot shows the quantification of the oxidation of arachidonic acid to malondialdehyde