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. 2020 May 7;10:7720. doi: 10.1038/s41598-020-63912-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the different chromosomal and plasmidic telomeres. (a) The terminal 180 nucleotide sequences from the 10 chromosomes and 2 linear plasmids were aligned. Four different groups designed in the table as telomere sub-clades were defined according to their sequence identities. Palindromic sequences are boxed and numbered in Arabic numerals above the sequences and the telomere loops are highlighted in yellow. Compensatory nucleotide changes within palindromes IV and VI of RLB3-7 telomere are highlighted in blue. The telomere sequence of plasmid pS1D4-20.1 could not be aligned with the others and is not presented. (b) Unrooted NJ phylogenetic tree built with the different telomere sequences. Positions with <80% site coverage in the alignment were eliminated enabling to have a total of 164 nucleotide positions in the final dataset. Bootstrap percentages are indicated on the branches. (c) The predicted secondary structures for a representative sequence of each telomere sub-cluster are represented. Two different loops (CTTG and GGA) can be observed at the top of the hairpin structures.