Fig. 2.

Deletion of HSP60 in adult cardiomyocytes results in mouse lethality and dilated cardiomyopathy. a Survival curves of control (n = 12) and HSP60^CKO (n = 16) mice after tamoxifen injection. Significance was determined by the Kaplan–Meier survival analysis. p < 0.001 versus control. b Representative hearts (top) and H&E stained sections (bottom) of control and HSP60^CKO mice 11 weeks post tamoxifen injection. c Ratios of ventricle weight to body weight (VW/BW) and lung weight to body weight (LW/BW) in control and HSP60^CKO mice 11 weeks post tamoxifen injection. n = 6 mice per group. d Masson’s trichrome staining of ventricular sections revealed increased fibrosis (blue) in HSP60^CKO hearts 11 weeks post tamoxifen injection. e Ratios of fibrosis area to the total cell area. n = 4 per group. f Immunostaining of cell apoptosis marker cleaved caspase 3 (red) in control and HSP60^CKO ventricular sections 11 weeks post tamoxifen injection. The sections were counterstained with DAPI (blue) to visualize the nucleus. g Ratios of cleaved caspase 3-positive nuclei to the total nuclei. n = 4 per group. All Data represent mean ± SEM. Significance was determined by two-tailed, unpaired Student’s t-test. **p < 0.01, ***p < 0.001 versus control