Fig. 6.

Assessment of mitochondrial unfolded protein response (UPR^mt) and mitochondrial protease expression in control and HSP60^CKO hearts. a Mitochondrial UPR^mt was assessed by quantitative RT-PCR analysis of mtHsp70 and Chop mRNA levels in control and HSP60^CKO hearts at 6 weeks (6w), 9 weeks (9w) and 11 weeks (11w) post tamoxifen injection. n = 6–7 mice per group. b, c Western blotting (b) and quantitative analysis (c) of mitochondrial proteases including CLPP, LONP1, and HTRA2 in control and HSP60^CKO hearts at 6 weeks, 9 weeks and 11 weeks post tamoxifen injection. n = 3–6 mice per group. All Data represent mean ± SEM. Significance was determined using the two-way ANOVA analysis with Bonferroni post-hoc test. *p < 0.05, ***p < 0.001 versus control