Fig. 1.

MAP3K13 increases Myc stability and transcriptional activity in HCC. a Protein levels of MAP3K13, TRIM25, FBXW7α and Myc in MAP3K13 shRNA-transfected HepG2 and FHCC98 cells were detected by immuno-blot. β-actin levels were used as a loading control. b Real-time qRT-PCR analysis of Myc expression in the above cells. Data were presented as the mean ± SD, *p < 0.05. c MAP3K13 regulates Myc turnover. HepG2 and FHCC98 cells were transfected with MAP3K13 shRNA or control vectors followed by cycloheximide (CHX) block and collected at the indicated times for immuno-blot. d The intensity of Myc expression for each time point in c was quantified by densitometry, with β-actin as a normalizer. Data were presented as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001. e Gene-set enrichment analysis (GSEA) of MAP3K13 in HCC patients from GSE102083 and GSE25097. Representative GSEA plots indicated that predefined gene sets involved in the Myc pathway were positively associated with high MAP3K13 expression. ES enrichment score