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. 2020 May 7;11(5):332. doi: 10.1038/s41419-020-2528-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Quantitative real-time PCR (qRT-PCR) analysis for mRNA expression levels of CRIF1 in five human HCC cell lines (SNU-354, SNU-368, SNU-739, HLE, and HLF) and one normal human hepatic cell line HL-7702. b Western blot analysis for protein expression levels of CRIF1 in five human HCC cell lines (SNU-354, SNU-368, SNU-739, HLE, and HLF) and one normal human hepatic cell line HL-7702. c Quantitative RT-PCR analysis for mRNA expression levels of CRIF1 in paired tumor and peritumor tissues from 30 HCC patients. (T tumor, P peritumor). d, e UALCAN (a portal for facilitating tumor subgroup gene expression and survival analyses) websites-based bioinformatic analysis of CRIF1 expression in HCC. f Immunohistochemical staining for expression levels of CRIF1 in paired tumor and peritumor tissues from 183 HCC patients. Left panel: representative immunohistochemical (IHC) staining images of CRIF1 in paired tumor and peritumor tissues of HCC. Scale bar, 50 μm. Right panel: IHC staining scores of CRIF1 in paired tumor and peritumor tissues of HCC. g, h Overall survival (OS) and recurrence-free survival (RFS) were evaluated by the Kaplan–Meier survival curves and the log-rank test in tumor tissues from 183 HCC patients according to CRIF1 expression levels. Data are expressed as mean ± SEM from three independent experiments. *p < 0.05.