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. 2020 May 7;11(5):332. doi: 10.1038/s41419-020-2528-7

Fig. 3. CRIF1 enhanced invasion and migration abilities of HCC cells through induction of epithelial–mesenchymal transition (EMT).

Fig. 3

a Cell migration ability was determined by wound healing assay in SNU-739 and SNU-354 cells with indicated treatment for 48 h (siCRIF1, siRNA against CRIF1; siCtrl, control siRNA; EV, empty vector; CRIF1, expression vector encoding CRIF1). Vertical line indicate boundary of cell wound, horizontal lines indicate scale bars, 50 μm. b Cell invasion ability was determined by the transwell matrigel invasion assay in SNU-739 and SNU-354 cells with indicated treatment for 48 h (Scale bars, 20 μm). c, d Quantitative RT-PCR and western blot analysis for expression levels of EMT markers (E-cadherin, ZO-1, N-cadherin, and vimentin) in SNU-739 and SNU-354 cells with indicated treatment for 48 h. Data are expressed as mean ± SEM from three independent experiments. *p < 0.05.