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. 2020 May 7;11(5):336. doi: 10.1038/s41419-020-2518-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b Quantitative real-time RT-PCR and western blot analysis of MUC15 expression level in human normal and renal cancer cell lines (N = 3). c, d Quantitative real-time RT-PCR and western blotting analysis of MUC15 mRNA expression in ACHN or Caki-1 cell lines transfected with MUC15 shRNAs and shControl, and 786-O cell line infected with MUC15 lentivirus and negative control. 18S was applied as the endogenous control for quantitative real-time RT-PCR, and GAPDH was used as a loading control for western blotting assay (N = 3).