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. 2020 May 7;11(5):336. doi: 10.1038/s41419-020-2518-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The heat map of protein expression of 278 RCC samples in TCGA database was analyzed and drawn by MORPHEUS. AKT, beta-Catenin, GSK3-alpha-beta, PTEN, RAD50, BRD4 protein expression level was elevated significantly in all tested proteins. b Real-time quantitative PCR analysis of MMP2 and MMP9 mRNA expression level in MUC15-KD ACHN and Caki-1 sublines, MUC15-OV 786-O sublines (N = 3). c Western blot analysis of MMP2, MMP9 and PI3K/AKT signaling in MUC15-KD ACHN and Caki-1 sublines, MUC15-OV 786-O sublines. 18S was applied as the endogenous control for quantitative real-time RT-PCR, and GAPDH was used as a loading control for western blotting assay (N = 3) (*P < 0.05, **P < 0.01, ***P < 0.001).