Skip to main content
. 2020 May 7;11(5):329. doi: 10.1038/s41419-020-2557-2

Fig. 2. SNORD3A specifically enhances the chemosensitivity of breast cancer cells to 5-FU.

Fig. 2

a The effects of SNORD3A overexpression on the chemosensitivity of MDA-MB-231 cell to 5-FU, cisplatin (cDDP), and paclitaxel (PTX) were detected by MTS assay at 72 h post-treatment. b, c Colony-formation assay (b) and soft agar colony-formation assay (c) were performed to evaluate the colony growth of MDA-MB-231 cells under treatment with 5-FU (100 μg/ml), cDDP (5 μg/ml), or PTX (10 ng/ml). Scale bar, 100 μm. d Western blot showing UMPS, DPD, TS, and MTHFR protein levels in MCF-7 and MDA-MB-231 cells with or without SNORD3A overexpression. e UMPS protein levels in MCF-7 and MDA-MB-231 cells with SNORD3A overexpression and UMPS knockdown were examined by western blot. f Knockdown of UMPS abrogated the effects of SNORD3A overexpression on the chemosensitivity of MDA-MB-231 cells to 5-FU by MTS assay at 72 h post-treatment. g, h Colony growth of MDA-MB-231 cells with simultaneous expression interference of SNORD3A and UMPS were detected by colony-formation assay (g) and soft agar colony-formation assay (h). Scale bar, 100 μm. Student’s t test, mean ± s.d. *p < 0.05, **p < 0.01, ***p < 0.001.