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. 2020 May 7;11(5):329. doi: 10.1038/s41419-020-2557-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Schematic illustration of the interaction between SNORD3A and miR-185-5p predicted with DIANA tools (http://carolina.imis.athena-innovation.gr/diana_tools/web/index.php) and between miR-185-5p and UMPS mRNA 3′-UTR predicted by TargetScan (http://www.targetscan.org/vert_72/). b The interaction between SNORD3A and miR-185-5p and the minimum free energy (mfe) were predicted with the RNAhybrid software (https://bibiserv.cebitec.uni-bielefeld.de/rnahybrid). c MS2-RIP assay followed by qRT-PCR to detect the association between SNORD3A and miR-185-5p. miR-191-5p served as a negative control. d Luciferase activity demonstrated that miR-185-5p targeted SNORD3A. e Western blot was performed to detect UMPS protein levels in MCF-7 and MDA-MB-231 cells after transfection with miR-185-5p inhibitor. f Luciferase activity indicated that miR-185-5p targeted UMPS. g miR-185-5p expression levels in human breast cancer cell lines were detected by qRT-PCR. h miR-185-5p levels in breast cancer tumor tissues and paired non-tumorous mammary tissues were examined by qRT-PCR (n = 26). Student’s t test, mean ± s.d. **p < 0.01, ***p < 0.001, ****p < 0.0001.