Fig. 5.

miR-200c plays an vital role in the downregulation of SOX2 protein by ChlA-F. a, b Overexpression of miR-200c in T24T cells was evaluated by real-time PCR, and SOX2 protein expression was determined by Western blot. c miR-200c inhibitor lentivirus was used to infect T24T cells, and knockdown efficiency was determined by real-time PCR. Results are presented as the mean ± SD of triplicates. The asterisk (*) indicates a significant decrease as compared to Vector control (p < 0.05). d T24T (Vector) and T24T (miR200c inhibitor) cells were treated with or without 8 µM ChlA-F. SOX2 protein expression was determined by Western blot. β-Actin was used as a protein loading control. e The invasion abilities of T24T (Vector) and T24T (miR200c inhibitor) cells treated with or without 8 µM ChlA-F were evaluated by using a BD BioCoat ^TM Matrigel ^TM Invasion Chamber. Migration ability was determined by using the empty insert membrane without Matrigel. Invasion ability was assessed by using the same system with Matrigel applied. The scale bar was 200 μM. f, g The invasion and migration rates were normalized with the insert control according to the manufacturer’s instructions. The asterisk (*) indicates a significant difference between cells treated with or without 8 µM ChlA-F