Fig. 1. HucMSC-Ex treatment reduced renal interstitial fibrosis.
a The size of hucMSC-Ex was measured by NTA. b The morphology of hucMSC-Ex was identified by TEM. c The images of hucMSC-Ex were detected by cryo-EM. d Western blot assay for exosomal markers CD9, CD63, and Alix in hucMSC-Ex. e CM-DiR labeled hucMSC-Ex (10 mg/kg) were intravenously administrated into 14d UUO rats. The distribution of CM-DiR labeled hucMSC-Ex in the kidney detected by IVIS imaging system. f Schematic of UUO induction and exosomes treatment. UUO rats were intravenously injected with exosomes (10 mg/kg/injection) on days 6, 9, and 12. The rats were sacrificed on day 14 for subsequent experiments (n = 10). g Representative images of HE staining, Sirius red and Masson staining of kidneys in 14d UUO rats treated with hucMSC-Ex. Bar = 100 μm.