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. 2020 May 7;11(5):327. doi: 10.1038/s41419-020-2510-4

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© The Author(s) 2020

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Fig. 2 — a YAP immunohistochemistry staining of UUO 7d, 14d kidney sections. Bar = 100 μm. b Quantification of YAP-positive nuclei per field of view from the a experiment. c Double immunofluorescent staining of YAP and α-SMA in the kidneys of control and UUO (at 7 and 14 days) rats (Green: YAP; Red: α-SMA). Bar = 50 μm. d Western blot assay for YAP protein expression in the kidneys of control and UUO rats (at 7 and 14 days) rats (n = 3). e Nuclear localization of YAP in NRK-52E cells treated with different stiff gel for 48 h was detected by confocal microscopy. Bar=25 μm. f Statistical analysis the expression of YAP in cytoplasm and nuclear under mechanical pressure. **P < 0.01.