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. 2020 May 7;11(5):327. doi: 10.1038/s41419-020-2510-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a YAP immunohistochemistry staining of 14d UUO kidney with hucMSC-Ex treatment. Bar=100 μm. b Quantification of YAP-positive nuclei per field of view from the a experiment. c Double immunofluorescent staining of YAP and α-SMA in the kidney of 14d UUO rats treated with hucMSC-Ex (Green: YAP; red: α-SMA). Bar = 50 μm. d Western blot analyses of YAP expression in the kidney of 14d UUO rats with hucMSC-Ex intervene (n = 3). e The co-localization of YAP and α-SMA in NRK-52E cells cultured under stiff gel condition in the presence of hucMSC-Ex was detected by double immunofluorescent staining. Bar=25 μm. f Statistical analysis the expression of YAP in cytoplasm and nuclear with hucMSC-Ex therapy. **P < 0.01.