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. 2019 Jun 19;27(1):379–395. doi: 10.1038/s41418-019-0361-2

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© The Author(s) 2019, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — DC-SIGN is frequently upregulated and its positive expression is associated with poor prognosis in CRC. a Transcripts expression of DC-SIGN in various human cancer and normal cell lines was detected by standard PCR (upper panel). The PCR products from LoVo cells were confirmed by sequencing (lower panel). b Expression of DC-SIGN protein in human CRC. HEK293 transfected with DC-SIGN vector, rhDC-SIGN-Fc, and lymph node lysates were used as positive controls. c Cell surface expression of DC-SIGN in colon cancer cell lines was examined by flow cytometry. Numbers listed were percentage of positive cells. Thick line, DC-SIGN; Thin line, isotype control. d Confocal microscopy to determine colocalization of DC-SIGN and CEA in colon cancer cells. e IHC staining of DC-SIGN and CD11c or cytokine 20 coexpression in CRC tissue. f Images shown are representative of DC-SIGN staining in primary (pCRC) and metastatic CRC (mCRC). Para, paracarcinoma; LNM, lymph node metastasis; DM, distant metastasis. g Soluble DC-SIGN (sDC-SIGN) levels in serum derived from CRC patients. h The correlation between the tissue and serum DC-SIGN expression in matched CRC patients. i Kaplan–Meier analysis of the overall survival and metastasis-free survival of CRC patients. Data, mean ± SD. **P < 0.01