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. 2020 May 7;10:7727. doi: 10.1038/s41598-020-64319-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PBMC proliferation with RBC treatment. Figures represent proliferation of (a) CD3 + cells, (b) CD4 + cells, and (c) CD8 + cells from a PBMC population as fold change of proliferation from loss of CFSE fluorescence (MFI), and percentage of (d) CD4 + cells or (e) CD8 + cells in a CD3 + population of PBMCs following treatment with nothing (PBMC), with PHA-P (PBMC + PHA), with PHA-P and oRBCs (PBMC + PHA + oRBC), or with PHA-P and ccRBCs (PBMC + PHA + ccRBC). Proliferation index presented as mean ± SD and percentages presented as box and whisker plots with median (n ≥ 6). PBMCs were analysed by flow cytometry after 5 days in culture at 37 °C with 5% CO₂. Data are statistically significantly different if *p < 0.05, **p < 0.01, or ***p < 0.001.