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. 2020 May 7;10:7727. doi: 10.1038/s41598-020-64319-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Secretion of cytokines from PBMCs following RBC treatment. Summary of cytokines in PBMC conditioned media from untreated PBMCs (PBMC), PBMCs treated with PHA-P (PBMC + PHA), PBMCs treated with PHA-P and oRBCs (PBMC + PHA + oRBC), and PBMCs treated with PHA-P and ccRBCs (PBMC + PHA + ccRBC) as measured by Bio-Plex. Data are presented as box and whisker plots with median concentration (n = 5). Data are statistically significantly different from PBMCs treated with PHA-P if *p < 0.05, **p < 0.01, ***p < 0.001, or ****p < 0.0001 and PBMC + PHA + oRBCs are statistically significantly different from PBMC + PHA + ccRBCs if ^#p < 0.05.