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. 2020 May 7;11(5):325. doi: 10.1038/s41419-020-2522-0

Fig. 3. JunB mediates VEGFA-induced angiogenic events in HRMVECs.

Fig. 3

a Western blot analysis of control and various time periods of VEGFA (40 ng/ml)-treated HRMVECs for the indicated proteins. b The effect of siControl, siPKCθ and siJunB (100 nM) on VEGFA (40 ng/ml)-induced JunB expression at 2 h and PKCθ phosphorylation at 10 min. The blot was sequentially reprobed for PKCθ, JunB, β-tubulin and α-tubulin levels to show the specificity and efficacy of the siRNA on its target and off target molecules. c Upper panel: Western blot analysis of JunB and β-tubulin levels to show the specificity and efficacy of siControl and siJunB (100 nM) in HRMVECs. Bottom panel: The effect of siControl and siJunB on VEGFA (40 ng/ml)-induced HRMVEC migration. d–f All the conditions were same as in (c) except that cells were treated with and without VEGFA (40 ng/ml) and DNA synthesis (d), sprouting (e) or tube formation (f) were measured. The bar graphs represent quantitative analysis of three independent experiments. The values are presented as mean ± SD. *p < 0.01 vs vehicle control or siControl; **p < 0.01 vs siControl + VEGFA. Scale bars in (e) and (f) are 50 and 200 μm, respectively.