Fig. 3.

RBM47 controls p21 expression indirectly in a p53-dependent manner. a, b After a preceding transfection (48 h) with either the control siRNA (siCon) or the RBM47 siRNA (siRBM47) HCT116 wild-type cells were exposed to γIR (a) or etoposide (b) and analyzed at the indicated times for expression of RBM47, p53, p21, and actin. Blots shown are representative blots of at least three independent experiments. c–e Quantitative Real-Time-PCR for the determination of p21 (c, e) and Puma (d) mRNA expression levels in p53-proficient (wild-type) (c, d) and p53-deficient (p53−/−) HCT116 cells (e). Cells were transfected with either the control siRNA or the RBM47 siRNA 48 h before they were irradiated. Total RNA was isolated 0, 4, and 24 h following their exposure to γIR and analyzed with transcript-specific probes from Life Technologies. Values shown in (c–e) are the mean of at least three independent experiments ± S.D