Fig. 2.

ATF3 promotes ferroptosis induced by FINs. a HT1080 cells expressing ATF3 or transfected with the empty vector (EV) were treated with 2.5 μM of erastin for 24 h, and subjected to western blotting. b Viable HT1080 cells treated with erastin for 24 h were counted after stained with Trypan blue. c Viable HT1080 cells were counted after treatments with erastin (5 μM), Ferr-1 (2 μM), and/or Z-VAD-FMK (10 μg/ml) for 24 h. d HT1080 cells treated with/without erastin for 6 h were stained with C11-BODIPY, and subjected to flow cytometry analysis. e ATF3-knockout (KO) or -wild-type (WT) RPE cells were treated with 2.5 μM of erastin for 24 h for western blotting. f Viable RPE cells treated with erastin for 24 h were counted. g Viable RPE cells were counted after treated with erastin (5 μM), Ferr-1 (2 μM), and/or Z-VAD-FMK (10 μg/ml) for 24 h. h RPE cells treated with/without erastin for 6 h were stained with C11-BODIPY for flow cytometry analysis