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Atg9 promotes the localization of the actin regulator Ena/VASP. Ablation of Atg9 changes the normally very obvious plasma membrane- and ring canal (arrowheads)-associated distribution of Ena in egg chambers of control and Atg9-3xHA rescued mutant animals (a). Decreased level of Ena protein is seen in western blots of Atg9 mutant ovaries compared with control and rescued animals (b). Scale bar in a represents 50 µm
