Fig. 2. The NSL complex and dBRD4 colocalize on endogenous promoters.

a Barplot of mean firefly signal normalized to Renilla signal and control RNAi (GST) of each knockdown and screen (two replicates). Source data are provided as a Source Data file. b Heatmaps and summary plots of NSL3¹³ and dBRD4²⁵ ChIP profiles. Log2 fold changes over input are plotted for all genes. Genes of both heatmaps are clustered and sorted based on NSL3 ChIP signal. p-value < 2.2e-16 for one-sided Fisher’s Exact Test on overrepresentation of dBRD4 binding on NSL3-bound promoters. c Scatterplot of NSL3¹³ and dBRD4²⁵ ChIP signals on gene promoters (TSS ± 200bp). Log2 fold changes over input are plotted for all gene promoters. R² was calculated with linear regression model. d Polytene chromosome immunostaining of daGal4 > UAS-fs(1)h-L-HA third instar larvae. NSL3 staining in red, HA staining in green: tagged long isoform of dBRD4 (dBRD4-L-HA). Scale bar: 5 µm. This experiment was repeated independently two more times showing similar results. e Gene Ontology (GO) Enrichment analysis of Biological Processes of genes promoter-bound by both dBRD4 and NSL3. Promoter-bound genes were defined by MACS peak calling on dBRD4²⁵ and NSL3¹³ ChIP-seq datasets considering TSS ± 200 bp as promoter region. Enriched GO terms were visualized with REVIGO.