Fig. 3.

PKM2 negatively regulates the levels of H2Bub1. a H1299, HEK293T, and HeLa cells were transfected with or without (-) Flag-tagged PKM2 at an increasing dosage for 48 h. Western blot analysis was then performed. b H1299, A549, and HEK293T cells were transfected with control or PKM2-specific shRNA or siRNA as indicated [14, 22, 42] for 48 h, followed by western blot analysis. c H1299 cells were transfected with or without (-) HA-tagged PKM1 at an increasing dosage for 48 h. Western blot analysis was then performed with antibodies as indicated. d H1299 cells were transfected with Flag-tagged wild-type PKM2 (Flag-PKM2-WT), pyruvate kinase-dead mutant PKM2 (Flag-PKM2-KD (K367M)), or Flag empty vector (Cont.) for 48 h. Western blot analysis was then performed using antibodies as indicated. e H1299 cells were transfected with control (shCont.) or PKM2-specific (shPKM2) shRNA for 48 h, followed by western blot analysis. All the statistical analyses of western blot are presented by mean with 95% CI. *p < 0.05, **p < 0.01, ***p < 0.001