Fig. 4.

PVT1 binds to KAT2A in NPC cells. a The RIP-qPCR assay of the binding of KAT2A with PVT1 in HNE-1 and CNE-1 cells. b Biotinylated PVT1 was incubated with extracts (HNE-1 and CNE-1 cells), treated with streptavidin beads, and binding proteins were resolved in a gel. The WB assay of the specific binding of KAT2A and PVT1. c Graphic illustration of predicted PVT1 secondary structure (http://www.lncipedia.org), and the truncation of PVT1 in accordance with the stem-loop structure. d Secondary structure regions of PVT1 were treated as in b, and binding KAT2A was detected by the WB assay. e, f, WB detection of KAT2A binding upon secondary structure regions of PVT1 after Flag-MS2bp-MS2bs-based pull-down assay. g Effects of PVT1 knockdown on the specific binding of KAT2A and PVT1. Error bars ± SD. **P < 0.01. ***P < 0.001. Data are representative from two independent experiments