Fig. 6.

KAT2A acetyltransferase activity is required for PVT1-driven HIF-1α stabilization. a Effects of PVT1 knockdown on KAT2A and the acetylation of H3K9 expression in HNE-1 and CNE-1 NPC cells. β-actin and Histone H3 were used as controls. b Effects of overexpression of KAT2A wild-type and acetyltransferase activity-deficient mutant, E570A/D610A, on KAT2A depletion-inhibited HIF-1α expression. c Overexpression of KAT2A wild type but not acetyltransferase activity-deficient mutant, E570A/D610A, restored PVT1 depletion-inhibited HIF-1α expression. Effects of overexpression of KAT2A wild-type and acetyltransferase activity-deficient mutant, E570A/D610A, on NPC cell proliferation (d) and colony formation (e). Inhibition of HIF-1α reduced KAT2A-induced HIF-1α protein expression (f), NPC cell proliferation (g), and colony formation (h). Error bars ± SD. **P < 0.01, ***P < 0.001. Data are representative from three independent experiments