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. 2019 Jul 18;27(2):695–710. doi: 10.1038/s41418-019-0381-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — PVT1 promotes TIF1β/H3K9ac complex-mediated NF90 transcriptional activation to regulate HIF-1α stability. Effects of PVT1 knockdown on NF90 protein (a) and mRNA (b) expression in HNE-1 and CNE-1 NPC cells. c Overexpression of KAT2A restored PVT1 knockdown-inhibited NF90 expression. d, e The ChIP-qPCR assay of the association of KAT2A or WDR5 with the NF90 promoter. After using antibodies against KAT2A, WDR5, or control IgG, qPCR assays were conducted using primers corresponding to five different loci of the NF90 promoter. Effects of NF90 knockdown on HIF-1α protein (f) and mRNA (g) expression in HNE-1 and CNE-1 NPC cells. Overexpression of NF90 restored PVT1 (h) and KAT2A (i) knockdown-inhibited NF90 expression. j Effects of overexpression of TIF1β wild-type and K554A/K575A mutant on PVT1 knockdown-inhibited HIF-1α, NF90, and the acetylation of H3K9 expression in HNE-1 cells. k Representation of overexpression of TIF1β wild type but not K554A/K575A mutant on PVT1 knockdown-inhibited the association of KAT2A or WDR5 with the NF90 promoter. Error bars ± SD. **P < 0.01. ***P < 0.001. Data are representative from three independent experiments