Fig. 9.

PVT1 suppresses the radiosensitivity of NPC cell lines by increasing the interaction between TIF1β and H3K9ac. a Effects of PVT1 overexpression in HNE-1 and CNE-1 NPC cells. Colony formation assays of PVT1 knockdown-(b) and overexpression-(c) mediated radiosensitivity in HNE-1 and CNE-1 NPC cells. Cell proliferation assays of PVT1 knockdown-(d) and overexpression-(e) mediated radiosensitivity in HNE-1 and CNE-1 NPC cells. f Representative bioluminescence images of PVT1 overexpression-restored radiation-inhibited HNE-1 subcutaneous tumor generation. Mice were imaged at 3–4 weeks after implantation. Data were from two independent experiments with five mice per group with similar results. g Quantification of the bioluminescence activity in f. h Representative bioluminescence images of PVT1 knockdown-induced radiation-inhibited HNE-1 subcutaneous tumor generation. i Quantification of the bioluminescence activity in h. j Overexpression of PVT1 rescues radiation-inhibited HIF-1α and NF90 expression. k The RIP assay of radiation has no effect on the association of KAT2A or WDR5, and PVT1. l Overexpression of PVT1 rescues radiation-inhibited the interaction between TIF1β and H3K9ac. m Effects of PVT1 overexpression on radiation-inhibited the association of KAT2A or WDR5 with the NF90 promoter. Error bars ± SD. *P < 0.05. **P < 0.01. ***P < 0.001. Data are representative from three independent experiments