Fig. 2.

Overexpression of Cebpd gene in mouse cerebellar granular cultures. a A significant increase in Cebpd mRNA expression is observed in pcDNA3-Cebpd granular cultures when compared with pcDNA3 cerebellar granular cultures (p < 0.0001). b C/EBPδ protein increases 12.2-fold (p < 0.0001) in pcDNA3-Cebpd cerebellar granular cultures. c A significant decrease in Snca mRNA expression is observed in pcDNA3-Cebpd cerebellar granular cultures when compared with pcDNA3 cerebellar granular cultures (p = 0.0003). d The 15 kDa band of α-synuclein protein is decreased in pcDNA3-Cebpd cerebellar granular cultures (p = 0.0062). White and black bars correspond to pcDNA3 and pcDNA3-Cebpd, respectively. Bars show means ± SD; n = 6 independent experiments. e Immunofluorescence shows a clear decrease in α-synuclein immunoreactivity in pcDNA3-Cebpd cerebellar granular cultures. Magnification bar, 100 µm. f Electroporation efficiencies of pcDNA3 and pcDNA3-Cebpd were 88.1 and 82.3%, respectively. g A significant decrease in α-synuclein levels (p < 0.0001) in the conditioned media of pcDNA3-Cebpd cerebellar granular cultures when compared with pcDNA3 cerebellar granular cultures was determined by ELISA. Cebpd and Snca mRNAs are evaluated in cerebellar granular cultures by qRT-PCR using Hprt and Rn18s as housekeeping genes. C/EBPδ and α-synuclein protein are assessed in cerebellar granular cultures by western blot using β-actin as the normalizing protein. **p < 0.01 and ***p < 0.001, using Student’s t-test