Fig. 3.

Overexpression of CEBPD gene in human SH-SY5Y cultures. a A significant increase in CEBPD mRNA expression (7.6-fold) is observed in SH-SY5Y cultures transfected with pcDNA3-CEBPD when compared with pcDNA3 (p < 0.0001). b A significant decrease in SNCA mRNA is detected (p = 0.0028) in pcDNA3-CEBPD transfected cultures. c A significant decrease in α-synuclein protein levels (p = 0.0098) in the conditioned media of SH-SY5Y cultures transfected with pcDNA3-CEBPD was determined by ELISA. d The 15 kDa band of α-synuclein protein is decreased in the pcDNA3-CEBPD SH-SY5Y cultures (p = 0.0002). In this bargraph bars show means ± SD of n = 6 independent experiments and white and black bars correspond to pcDNA3 and pcDNA3-CEBPD, respectively. CEBPD and SNCA mRNAs are evaluated in SH-SY5Y cultures by qRT-PCR using ACTB and HPRT1 as the housekeeping genes. α-Synuclein protein is evaluated in SH-SY5Y cultures by western blot using β-actin as normalizing protein. **p < 0.01 and ***p < 0.001, using Student’s t-test