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. 2020 May 7;10:7704. doi: 10.1038/s41598-020-64737-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MCR–ALS Analysis of the Raman hyperspectral imaging data of the pink biofilm assuming three components (k = 3). (A) Intrinsic Raman spectra of the three components (denoted 1–3). Each spectrum has been normalized so that the sum of intensities at all Raman shifts is equal to unity. The spectra are vertically offset for clarity of display. (B–D) Raman images of components 1 (B), 2 (C), and 3 (D). The Raman images are shown in pseudocolor. (E) Merged image of B–D, indicating mutually exclusive distributions of components 1 (magenta) and 2 (green). Colocalization of components 2 (green) and 3 (red) is manifested as a yellow region (indicated by an arrow).