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. 2019 Oct 28;27(5):1633–1643. doi: 10.1038/s41418-019-0447-x

Fig. 5.

Fig. 5

Regulation of Mfn2 expression by HuD. Mfn2 mRNA and protein levels were analyzed in βTC6 cells transfected with siHuD (a) or βTC6-shHuD (b) cells by RT-qPCR and western blot analysis, respectively. c Schematic representation of EGFP reporter constructs containing Mfn2 mRNA 3′UTRs (pEGFP-Mfn2-3U1 and -3U2). d Following sequential transfection with a reporter plasmid after HuD knockdown in βTC6 cells, the relative EGFP expression was determined by measuring the fluorescence of EGFP and western blot analysis. Data represent the mean ± SEM from three independent experiments. **p < 0.01; ***p < 0.001