Fig. 5.

Regulation of Mfn2 expression by HuD. Mfn2 mRNA and protein levels were analyzed in βTC6 cells transfected with siHuD (a) or βTC6-shHuD (b) cells by RT-qPCR and western blot analysis, respectively. c Schematic representation of EGFP reporter constructs containing Mfn2 mRNA 3′UTRs (pEGFP-Mfn2-3U1 and -3U2). d Following sequential transfection with a reporter plasmid after HuD knockdown in βTC6 cells, the relative EGFP expression was determined by measuring the fluorescence of EGFP and western blot analysis. Data represent the mean ± SEM from three independent experiments. **p < 0.01; ***p < 0.001