Fig. 4.

FNDC5 activated AKT/mTOR signaling pathway in vivo and in vitro. a, b Western blots and quantitative results in murine hearts (n = 6). c, d Western blots and statistical results in cultured H9C2 cells (n = 6). Values represent the mean ± SEM. In figure (a, b), *P < 0.05 versus the corresponding normal saline (NS) group mice injected with negative control (NC) adeno-associated virus 9, ^#P < 0.05 versus DOX-treated mice injected with AAV9-NC. In figure (c, d), *P < 0.05 versus PBS group treated with control vehicle, ^#P < 0.05 versus DOX-treated H9C2 cells with irisin protection