Fig. 6.

AKT/GSK3β/FYN/Nrf2 signaling was responsible for FNDC5-mediated protective role on oxidative damage. a, b Western blots and statistical results (n = 6). c Relative mRNA levels of Nqo1, Gclc, and Gclm in cultured H9C2 cells (n = 6). d, e Keap1 protein levels in total cell lysates and Nrf2 protein levels in nuclear lysates (n = 6). f Relative Nrf2 mRNA level (n = 6). g, h Representative western blots (n = 6). i FYN protein levels in nuclear lysates (n = 6). j Efficiency of si Nrf2 determined by western blots (n = 6). k DCFH-DA staining and the statistical data (n = 6). l–o Western blots and quantitative results (n = 6). p Cell viability detected by CCK-8 assay (n = 6). Values represent the mean ± SEM. *P < 0.05 versus the matched group. NS means no significance