| sgRNA | CRISPR Sequence (5′→ 3′) | TIDE Primer Sequence (forward): (5′→ 3′) | TIDE Primer Sequence (reverse): (5′→ 3′) | Knockdown efficiency (TIDE) | Knockdown efficiency (Western Blot) |
|---|---|---|---|---|---|
| Nontargeting sgRNA | CTGTCTTCAACGTCTGGCCG | ||||
| SLC7a11 sgRNA#1 | GGACCAAGAGCCACCTGGGC | TGTAGAGCCAGTCGGTGATAGC | GGGTAGTGCACATACCTGAACAAC | 52.6% | 46% |
| SLC7a11 sgRNA#2 | GATGTAGCGTCCAAATGCCA | CCTCAAACCTTGTGTTCCTGTCTG | CTGGATTGCTATCTTCACAGGCC | 49.5% | 59% |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.