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. 2019 Sep 30;27(3):919–933. doi: 10.1038/s41418-019-0423-5

Fig. 3.

Fig. 3

circUHRF1 acts as the sponge of miR-526b-5p in OSCC cells. a Online bioinformatics tools CircInteractome (https://circinteractome.nia.nih.gov/) indicated the miR-526b-5p target for circUHRF1. b The wild-type and mutant sequences were constructed corresponding with the miR-526b-5p for the luciferase reporter assay. c The luciferase activity in the cotransfection of miR-526b-5p and circUHRF1 wild type or mutant. d Biotinylated-circUHRF1 probe was performed for RNA pull-down assay. RT-PCR revealed the miR-526b-5p expression in the pulled down SCC25 cells. e RT-PCR indicated the miR-526b-5p level in the SCC25 cells with transfection of circUHRF1 shRNA. f RT-PCR illustrated the miR-526b-5p level in the OSCC (SCC25, CAL27) cells. g RNA-FISH indicated the circUHRF1 and miR-526b-5p subcellular location. h The Pearson correlation analysis indicated the negative correlation within miR-526b-5p and circUHRF1. **p-value < 0.01