Fig. 4.

c-Myc functions as the target of circUHRF1/miR-526b-5p. a The c-Myc wild type and mutant sequence at 3′-UTR and miR-526b-5p were constructed. b Luciferase reporter assay indicated the luciferase activity after the cotransfection with c-Myc wild type or mutant and miR-526b-5p mimics or controls. c RT-PCR indicated the c-Myc mRNA after miR-526b-5p mimics transfection or miR-526b-5p inhibitor transfection. d, e Western blot analysis illustrated the c-Myc protein in OSCC cells (SCC25, CAL27) with miR-526b-5p mimics or inhibitor transfection. f, g Western blot analysis indicated the c-Myc protein in the transfection of circUHRF1 shRNA with miR-526b-5p inhibitor. **p-value  < 0.01