Fig. 1.

In vivo SVZ and OB neurogenesis is affected in Tg2576 mice. a Immunofluorescence staining for BrdU, progenitors, and neuroblasts markers in adult SVZ of Tg2576 and wild type (WT) mice. Immunostaining for incorporated BrdU in progenitor cells shows reduced proliferation in Tg2576 SVZ niche (red signal in top panels), while there is a significant reduction of aNSCs compartment (GFAP⁺ and Sox2⁺ cells) and an increase in DCX⁺ neuroblasts. Scale bar 100 µm, ×20 magnification (for BrdU) and 50 µm, ×40 magnification, zoom 1.3 (for SVZ markers). The histograms represent the quantification of BrdU (b) or Sox2, GFAP, and DCX positive cells (c) in Tg2576 (red) and WT (blue) SVZ. Data are means  ± SEM of five individual animals (n = 5) for each experimental group. **P < 0.01, ***P < 0.001, significantly different from WT, Mann–Whitney test. d Immunostaining for BrdU and NeuN (red and green, respectively, top panels, ×40 magnification) and BrdU and Calretinin (red and green, respectively, bottom panels, ×63 magnification) shows a significant reduction of newborn Calretinin interneurons in Tg2576 olfactory bulbs compared with WT. Scale bar 50 µm. e Quantification of double positive cells in Tg2576 (red) and WT (blue). Data are means  ± SEM of five individual animals (n = 5) for each experimental group. *P < 0.05, ***P < 0.001, significantly different from WT, Mann–Whitney test