Fig. 6.
Ectopic expression of AMBRA1ActA fusion gene impairs colony formation and MCL1 expression in FLT3-ITD-mutated AML CD34+ cells. a Colony-formation assay (CFU-assay) shows reduced colony-formation capacity after 15 days in culture in AMBRA1ActA (+Retro-AMBRA1ActA) overexpressing CD34+ cells, compared with noninfected (Untreated; t-test P = 0.0068) and empty vector infected (+Retro IRES BFP; t-test P = 0.0028) CD34+ cells. (Four technical replicates are shown). Histograms show the median number of colonies and SDs for the three conditions mentioned above. Difference between untreated and +Retro IRES BFP conditions is not statistically significant (t-test P = 0.41). b Western blot shows expression of ectopic AMBRA1ActA fusion gene in +Retro-AMBRA1ActA infected CD34+ cells along with MCL1 reduction compared with controls cells after 15 days in culture