Fig. 6.

Role of RAF kinases in moDC migration. a F-actin of LPS-stimulated moDCs, which were treated with LY3009120 (1 µM) or trametinib (1 µM), were stained with phalloidin and visualized by a Leica SP8 confocal microscope. b Morphology was analyzed by determining the circularity index of 26–60 cells per condition. c CCR7 mRNA levels were determined by real-time PCR after treating moDCs for 48 h with LY3009120 (1 µM) or trametinib (1 µM) in the presence of LPS (n = 4). d The surface marker expression of CCR7 was analyzed by flow cytometry after treating moDCs with LY3009120 (1 µM) or trametinib (1 µM) while stimulating with LPS (100 ng/ml) and PGE₂ (1 µg/ml) (n = 6). e Directed migration of moDCs towards the cytokine CCL21 (200 ng/ml) was determined in transwell migration experiments by counting successfully migrated cells after 3 h (n = 5)