Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 4;27(5):1588–1603. doi: 10.1038/s41418-019-0442-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2019

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Low glutamate concentrations cause mitochondrial depolarization in gba1^+/− and gba1^−/− neurons. a The mitochondrial membrane potential was measured using rhodamine 123 (with the ‘dequench protocol’) in responses to exposure to 10 μM glutamate stimulation and plotted as a function of time for each genotype gba1^+/+, gba1^+/−, and gba1^−/−. An increase in Rhod123 fluorescence intensity reports mitochondrial depolarization. The responses to 10 μM glutamate were significantly different between gba1^−/− and gba1^+/+ or gba1^+/− genotypes, revealing a large depolarization in the gba1^−/− and in gba^+/− cells (n = 3 biological replicates, N = 15–25 cells per genotype per experiment). b Scatter plots of the mitochondrial depolarization at 360 s after 10 μM glutamate stimulation, expressed as Rhod123 F/F₀ percentage comprised between the baseline (0%) and maximal fluorescence intensity value (100%) for the different genotypes. Average values are expressed as mean ± SD (left panel). Relative cumulative frequency distribution of the mitochondrial depolarization is also shown (right panel), showing a significant mitochondrial depolarization in response to 10 μM glutamate in gba1^+/− or gba1^−/− compared with gba1^+/+ (Kruskal–Wallis test, Dunns post-test, *p < 0.05)