Fig. 5.

Mitochondrial calcium buffering capacity is reduced and MCU protein level is decreased in gba1^−/− and gba1^+/− neurons. a Mitochondria-target aequorin plate reader assay was used to measure mitochondrial Ca²⁺ uptake in mixed neuronal and astrocytic cultures from gba1^+/+, gba1^+/−, and gba1^−/− in response to 10 μM glutamate. The data show that mitochondrial Ca²⁺ uptake was significantly reduced in both gba1^+/− and gba1^−/− cells compared with gba1^+/+ (n = 3–5 cultures per genotype, one-way Anova, post-hoc Bonferroni). b Protein expression levels of MCU complex components and MCU regulatory proteins evaluated by western blot in gba1^+/+, gba1^+/−, and gba1^−/− brains (data shown as scatter plots and mean ± SEM, n = 3–5 brains per genotype). MCU expression was significantly downregulated in gba1^+/− and gba1^−/− tissue (One-way Anova, post-hoc Bonferroni, *p < 0.05), while EMRE, MICU2, and MCUR1 expression levels were unchanged