Skip to main content
. 2020 May 7;11(5):335. doi: 10.1038/s41419-020-2549-2

Fig. 3. NT5DC2 upregulation did not affect apoptosis but facilitated cell cycle transition from the G1 to S phase in HCC cells; NT5DC2 knockdown reversed this process.

Fig. 3

a Flow cytometry for detecting apoptotic rates in MHCC97H-NT5DC2-overexpressing and PLC/RLF/5-NT5DC2-overexpressing cells compared with their vector controls. NT5DC2 did not affect the apoptotic rates in these two cell lines. b Flow cytometry for detecting apoptotic rates in MHCC97H-NT5DC2-knockdown and PLC/RLF/5-NT5DC2-knockdown cells compared with their vector controls. NT5DC2 knockdown did not affect the apoptotic rates in liver cancer cells. c Flow cytometry for detecting cell cycle ratios in MHCC97H-NT5DC2-overexpressing and PLC/RLF/5-NT5DC2-overexpressing cells compared with their vector controls (left). Statistical analysis of cell cycle phase ratios in MHCC97H-NT5DC2-overexpressing and PLC/RLF/5-NT5DC2-overexpressing cells (right). *p < 0.05; **p < 0.01; ***p < 0.001. d Flow cytometry for detecting cell cycle ratios of MHCC97H-NT5DC2-knockdown and PLC/RLF/5-NT5DC2-knockdown cells compared with their vector controls (left). Statistical analysis of cell cycle phase ratios in MHCC97H-NT5DC2-knockdown and PLC/RLF/5-NT5DC2-knockdown cells (right). *p < 0.05; **p < 0.01; ***p < 0.001. e Western blot analysis of the effects of NT5DC2 overexpression on the expressions of cell cycle-regulating proteins cyclin D1, cyclinD3, CDK4, CDK6, p18, p21, and p27. f Western blot analysis of the effects of NT5DC2 knockdown on the expressions of cell cycle-regulating proteins cyclin D1, cyclinD3, CDK4, CDK6, p18, p21, and p27.