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. 2019 Jul 1;27(2):646–661. doi: 10.1038/s41418-019-0378-6

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© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — EMC3, EMC5, and EMC6 were not required for maturation of the Rh1 protein. a Rh1 localized to the rhabdomere region in emc3, emc5, and emc6 mutant photoreceptor cells. Tangential resin-embedded retina sections of compound eyes from ~1-day-old wt, emc3^G7, emc3^G7;ninaE-emc3, emc5^G2, emc5^G2;ninaE-emc5, emc6^N10, and ninaE-emc6;emc6^N10 flies were labeled using antibodies against Rh1. Rhabdomeres are indicated by arrows. Scale bar is 2 μm. Illustration of photoreceptor cells within a single ommatidium is on the top right corner, and rhabdomeres of R1-R7 photoreceptor cells are indicated by numbers. b Deglycosylation of Rh1 is normal in emc3, emc5, and emc6 mutants. Head extracts prepared from 1-day-old wild-type, ninaA, dmppe^e02905, emc3^G7, emc5^G2, and emc6^N10 flies were probed with antibodies against Rh1, and mature and immature bands are indicated. Extracts from ninaA and emc6^N10 flies (10 heads), emc3^G7 and emc5^G2 flies (5 heads), and wild-type and dmppe^e02905 flies (0.25 of a head) were loaded. c–e Similar Rh1 levels were seen in (c) emc3^G7 and emc3^G7;hrd1¹ flies (ey-flp rh1-GFP;emc3^G7 FRT40A/GMR-hid CL FRT40A;FRT82B hrd1¹/FRT82B GMR-hid CL), d emc5^G2 and emc5^G2;hrd1¹ flies (ey-flp rh1-GFP;emc5^G2 FRT40A/GMR-hid CL FRT40A;FRT82B hrd1¹/FRT82B GMR-hid CL), and e emc6^N10 and emc6^N10 hrd1¹ (ey-flp rh1-GFP; FRT82B emc6^N10 hrd1¹/FRT82B GMR-hid CL) flies. Protein extracts from 0.25 of a head from ~1-day-old flies were loaded