Fig. 2.

CPAP promotes cancer cell and HUVEC migration. a, b HCC cells stably expressing GFP, GFP-CPAP, or knocked-down CPAP by pSUPER-SP1 or pSUPER-SP5 were used to perform Transwell migration assays (a) or invasion assay (b). pSUPER-NS2 is a negative control of shRNA. c Conditioned media from IL-6-treated Hep3B (Hep3BST) and HepG2 (HepG2ST) cells stably expressing GFP or GFP-CPAP were collected to perform HUVEC migration assays. d Hep3B/GFP-CPAP or Huh7/GFP-CPAP stable cells were starved and treated without (–) or with IL-6 or TNF-α for 24 h, and then cell migration (left) and invasion (right) assays were performed. The number of migrated or invasive cells was counted, and the quantitative results are shown. Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001