Fig. 2.

TNBS-induced fibrotic model demonstrated the enhancement of plasminogen activator inhibitor-1 (Pai-1) expression. BALB/c mice were intrarectal injected with 2% TNBS. (A) Representative histological examples of each group (H&E and Masson trichrome staining). (B) Collagen deposition was determined by the Sircol collagen assay. (C) The mRNA expression of murine Pai-1 in the colon samples was assessed by real-time PCR. Expression of the Pai-1 gene was normalized against that of the Gapdh gene in the same RNA preparation (5 ETOH, 10 TNBS). Bars represent the median. P-value by Mann-Whitney U-test. ^a P<0.05; ^b P<0.01. TNBS, 2,4,6-trinitrobenzene sulfonic acid; ETOH, ethanol.