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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Nat Protoc. 2020 Jan 8;15(2):628–648. doi: 10.1038/s41596-019-0263-2

Example biotin-conjugated lineage antibody master mix

Reagent Volume (μl)
Biotin-Gr1/Ly-6G (0.5 mg/mL) 100
Biotin-Ter119 (0.5 ml/mL) 100
Biotin-CD4 (0.5 mg/mL) 25
Biotin-CD8 (0.5 mg/mL) 25
Biotin-CD45R/B220 (0.5 mg/mL) 50
Biotin-CD127 (0.5 mg/mL) 50
Sterile PBS 350