Example antibody stain for 10 million c-Kit-enriched bone marrow cells
| Reagent | Volume (μl) |
|---|---|
| FITC-CD34 (0.5 mg/mL) | 4 |
| APC-c-Kit (0.2 mg/mL) | 1 |
| PE-Sca1 (0.2 mg/mL) | 1 |
| APC/eFluor780-Streptavidin (0.2 mg/mL) | 1 |
| PE/Cy7-CD150 (0.2 mg/mL) | 1 |
| Sterile PBS | 392 |
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